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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- NBP2-29973 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TRAF-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAF-2 Antibody (214CT16.3.4) [NBP2-29973] - T47D cell line lysates (35ug/lane).This demonstrates the TRAF2/MB10188 antibody detected the TRAF2/MB10188 protein (arrow).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAF-2 Antibody (214CT16.3.4) [NBP2-29973] - Anti- at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody (214CT16.3.4) [NBP2-29973] - TRAF2 Antibody (NBP2-29973) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
