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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB6989 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6989, RRID:AB_1581463
- Product name
- TRAF2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of TRAF2.
- Antigen sequence
C-KMEAKNSYVRDD- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Poly(ADP-ribose) polymerase-1 signaling to mitochondria in necrotic cell death requires RIP1/TRAF2-mediated JNK1 activation.
Xu Y, Huang S, Liu ZG, Han J
The Journal of biological chemistry 2006 Mar 31;281(13):8788-95
The Journal of biological chemistry 2006 Mar 31;281(13):8788-95
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TRAF2 polyclonal antibody (Cat # PAB6989) (0.1 ug/mL) staining of human ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing human TRAF2 and probed with TRAF2 polyclonal antibody (Cat # PAB6989) (mock transfection in first lane), tested by Origene.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TRAF2 polyclonal antibody (Cat # PAB6989)(3.8 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
