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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09417PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09417PU-N, RRID:AB_2036045
- Product name
- anti TRAF2 / TRAP3
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to an internal region human TRAF2
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.93 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-TRAF2 antibody shows detection of endogenous TRAF2 in whole HeLa cell lysates. Lane 2 shows endogenous TRAF2 detected with antibody at 47 kDa (arrowhead). Lane 4 shows no reactivity when blot is incubated with immunizing peptide. The identity of lower molecular weight band in lane 2 is unknown. Briefly, each lane contains approximately 14 µg of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1:500 dilution in 3% BSA-TBS and reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution conjugated Gt-a-Rabbit DyLight(TM)649 for 1 hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers in lanes 1 and 3. Fluorescence image was captured using the VersaDoc(R) Imaging System developed by Bio-Rad. Other detection systems will yield similar results.
