Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-14805 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDHA1 Monoclonal Antibody (S.975.6)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- S.975.6
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Oxygen regulates epithelial stem cell proliferation via RhoA-actomyosin-YAP/TAZ signal in mouse incisor.
Otsu K, Ida-Yonemochi H, Ikezaki S, Ema M, Hitomi J, Ohshima H, Harada H
Development (Cambridge, England) 2021 Feb 15;148(4)
Development (Cambridge, England) 2021 Feb 15;148(4)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), Neuro-2a (Lane 2), tissue extracts of Mouse Liver (Lane 3) and Mouse Heart (Lane 4). The blot was probed with Anti-PDH Monoclonal Antibody (Product # MA5-14805, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 40 kDa band corresponding to PDH was observed across the cell lines and tissues tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Pyruvate Dehydrogenase in extracts from various cell lines using Pyruvate Dehydrogenase monoclonal antibody (Product # MA5-14805).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), Neuro-2a (Lane 2), tissue extracts of Mouse Liver (Lane 3) and Mouse Heart (Lane 4). The blot was probed with Anti-PDH Monoclonal Antibody (Product # MA5-14805, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 40 kDa band corresponding to PDH was observed across the cell lines and tissues tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PDH was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PDH Monoclonal Antibody (S.975.6) (Product # MA5-14805) at 1:250 dilution in 0.1% BSA, incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Pyruvate Dehydrogenase in paraffin-embedded human lung carcinoma using a Pyruvate Dehydrogenase monoclonal antibody (Product # MA5-14805).