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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00005819-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005819-M01, RRID:AB_537482
- Product name
- Mouse Monoclonal Nectin-2/CD112 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PVRL2 - poliovirus receptor-related 2 (herpesvirus entry mediator B)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nectin-2/CD112 Antibody (2A6-2C1) [H00005819-M01] - Analysis of PVRL2 expression in transfected 293T cell line by PVRL2 monoclonal antibody (M01), clone 2A6-2C1.Lane 1: PVRL2 transfected lysate (Predicted MW: 51.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Nectin-2/CD112 Antibody (2A6-2C1) [H00005819-M01] - Detection limit for recombinant GST tagged PVRL2 is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Nectin-2/CD112 Antibody (2A6-2C1) [H00005819-M01] - Analysis of PVRL2 transfected lysate using anti-PVRL2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PVRL2 MaxPab rabbit polyclonal antibody.
