NBP1-85357

antibody from Novus Biologicals

Targeting: POR CYPOR, FLJ26468

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85357

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85357, RRID:AB_11010201

Product name

Rabbit Polyclonal POR/Cytochrome P450 Reductase Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat POR/Cytochrome P450 Reductase antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Analysis in human cell line SK-BR-3.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human liver shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human skeletal muscle shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Analysis in human liver and skeletal muscle tissues. Corresponding POR RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human liver shows very strong granular cytoplasmic positivity in hepatocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.