NBP2-32096

antibody from Novus Biologicals

Targeting: SELENOS AD-015, MGC2553, SBBI8, SELS, SEPS1, VIMP

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP2-32096

Provider

Novus Biologicals

Product name

Rabbit Polyclonal SELS Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SELS Antibody [NBP2-32096] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-Selenoprotein S antibody NBP2-32096 used for IP at 6 ug per reaction. Selenoprotein S was also immunoprecipitated by rabbit anti-Selenoprotein S antibody BL16285. For blotting immunoprecipitated Selenoprotein S, NBP2-32096 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SELS Antibody [NBP2-32096] - Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Selenoprotein S antibody NBP2-32096 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.