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- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB2365A - Provider product page
- Provider
- R&D Systems
- Product name
- Human TIM-3 APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TIM-3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM-1, rhTIM-4, recombinant mouse (rm) TIM-1, rmTIM-2, rmTIM-3, rmTIM-5, or rmTIM-6.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Red dye
- Antigen sequence
Q8TDQ0.2- Isotype
- IgG
- Antibody clone number
- 344823
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references CD226 opposes TIGIT to disrupt Tregs in melanoma.
Antitumor Activity Associated with Prolonged Persistence of Adoptively Transferred NY-ESO-1 c259T Cells in Synovial Sarcoma.
T-cell invigoration to tumour burden ratio associated with anti-PD-1 response.
Circulating T lymphocyte subsets, cytokines, and immune checkpoint inhibitors in patients with bipolar II or major depression: a preliminary study.
TIM-3/Gal-9 interaction induces IFNγ-dependent IDO1 expression in acute myeloid leukemia blast cells.
Inhibitory phenotype of HBV-specific CD4+ T-cells is characterized by high PD-1 expression but absent coregulation of multiple inhibitory molecules.
Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy.
Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
Blockade of Tim-3 signaling restores the virus-specific CD8⁺ T-cell response in patients with chronic hepatitis B.
Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection.
Fourcade J, Sun Z, Chauvin JM, Ka M, Davar D, Pagliano O, Wang H, Saada S, Menna C, Amin R, Sander C, Kirkwood JM, Korman AJ, Zarour HM
JCI insight 2018 Jul 26;3(14)
JCI insight 2018 Jul 26;3(14)
Antitumor Activity Associated with Prolonged Persistence of Adoptively Transferred NY-ESO-1 c259T Cells in Synovial Sarcoma.
D'Angelo SP, Melchiori L, Merchant MS, Bernstein D, Glod J, Kaplan R, Grupp S, Tap WD, Chagin K, Binder GK, Basu S, Lowther DE, Wang R, Bath N, Tipping A, Betts G, Ramachandran I, Navenot JM, Zhang H, Wells DK, Van Winkle E, Kari G, Trivedi T, Holdich T, Pandite L, Amado R, Mackall CL
Cancer discovery 2018 Aug;8(8):944-957
Cancer discovery 2018 Aug;8(8):944-957
T-cell invigoration to tumour burden ratio associated with anti-PD-1 response.
Huang AC, Postow MA, Orlowski RJ, Mick R, Bengsch B, Manne S, Xu W, Harmon S, Giles JR, Wenz B, Adamow M, Kuk D, Panageas KS, Carrera C, Wong P, Quagliarello F, Wubbenhorst B, D'Andrea K, Pauken KE, Herati RS, Staupe RP, Schenkel JM, McGettigan S, Kothari S, George SM, Vonderheide RH, Amaravadi RK, Karakousis GC, Schuchter LM, Xu X, Nathanson KL, Wolchok JD, Gangadhar TC, Wherry EJ
Nature 2017 May 4;545(7652):60-65
Nature 2017 May 4;545(7652):60-65
Circulating T lymphocyte subsets, cytokines, and immune checkpoint inhibitors in patients with bipolar II or major depression: a preliminary study.
Wu W, Zheng YL, Tian LP, Lai JB, Hu CC, Zhang P, Chen JK, Hu JB, Huang ML, Wei N, Xu WJ, Zhou WH, Lu SJ, Lu J, Qi HL, Wang DD, Zhou XY, Duan JF, Xu Y, Hu SH
Scientific reports 2017 Jan 11;7:40530
Scientific reports 2017 Jan 11;7:40530
TIM-3/Gal-9 interaction induces IFNγ-dependent IDO1 expression in acute myeloid leukemia blast cells.
Folgiero V, Cifaldi L, Li Pira G, Goffredo BM, Vinti L, Locatelli F
Journal of hematology & oncology 2015 Apr 16;8:36
Journal of hematology & oncology 2015 Apr 16;8:36
Inhibitory phenotype of HBV-specific CD4+ T-cells is characterized by high PD-1 expression but absent coregulation of multiple inhibitory molecules.
Raziorrouh B, Heeg M, Kurktschiev P, Schraut W, Zachoval R, Wendtner C, Wächtler M, Spannagl M, Denk G, Ulsenheimer A, Bengsch B, Pircher H, Diepolder HM, Grüner NH, Jung MC
PloS one 2014;9(8):e105703
PloS one 2014;9(8):e105703
Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy.
Meggyes M, Miko E, Polgar B, Bogar B, Farkas B, Illes Z, Szereday L
PloS one 2014;9(3):e92371
PloS one 2014;9(3):e92371
Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
Wang JM, Shi L, Ma CJ, Ji XJ, Ying RS, Wu XY, Wang KS, Li G, Moorman JP, Yao ZQ
Journal of virology 2013 Apr;87(8):4372-83
Journal of virology 2013 Apr;87(8):4372-83
Blockade of Tim-3 signaling restores the virus-specific CD8⁺ T-cell response in patients with chronic hepatitis B.
Wu W, Shi Y, Li S, Zhang Y, Liu Y, Wu Y, Chen Z
European journal of immunology 2012 May;42(5):1180-91
European journal of immunology 2012 May;42(5):1180-91
Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection.
Zhang Y, Ma CJ, Wang JM, Ji XJ, Wu XY, Jia ZS, Moorman JP, Yao ZQ
PloS one 2011;6(5):e19664
PloS one 2011;6(5):e19664
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TIM-3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM-3 APC-conjugated Monoclonal Antibody (Catalog # FAB2365A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
