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- Antigen structure
- References [25]
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- Product number
- FAB2365P - Provider product page
- Provider
- R&D Systems
- Product name
- Human TIM-3 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TIM-3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM-1, rhTIM-4, recombinant mouse (rm) TIM-1, rmTIM-2, rmTIM-3, rmTIM-5, or rmTIM-6.
- Reactivity
- Human
- Host
- Rat
- Antigen sequence
Q8TDQ0.2- Isotype
- IgG
- Antibody clone number
- 344823
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors.
Phase I trial of plerixafor combined with decitabine in newly diagnosed older patients with acute myeloid leukemia.
PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells.
Synergistic effect of IL-12 and IL-18 induces TIM3 regulation of γδ T cell function and decreases the risk of clinical malaria in children living in Papua New Guinea.
TCR-ligand dissociation rate is a robust and stable biomarker of CD8+ T cell potency.
Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE.
Dendritic cell vaccination in combination with docetaxel for patients with metastatic castration-resistant prostate cancer: A randomized phase II study.
BCG vaccination induces HIV target cell activation in HIV-exposed infants in a randomized trial.
Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9.
CD3(bright)CD56(+) T cells associate with pegylated interferon-alpha treatment nonresponse in chronic hepatitis B patients.
CD62L+ NKT cells have prolonged persistence and antitumor activity in vivo.
Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition.
Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection.
CD127 expression, exhaustion status and antigen specific proliferation predict sustained virologic response to IFN in HCV/HIV co-infected individuals.
Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy.
Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection.
Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
The immunomodulatory effects of bevacizumab on systemic immunity in patients with metastatic melanoma.
Age-related expansion of Tim-3 expressing T cells in vertically HIV-1 infected children.
Tim-3 negatively regulates cytotoxicity in exhausted CD8+ T cells in HIV infection.
Extended co-expression of inhibitory receptors by human CD8 T-cells depending on differentiation, antigen-specificity and anatomical localization.
Upregulation of the Tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
Antigen-independent induction of Tim-3 expression on human T cells by the common γ-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway.
Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis.
Duhen T, Duhen R, Montler R, Moses J, Moudgil T, de Miranda NF, Goodall CP, Blair TC, Fox BA, McDermott JE, Chang SC, Grunkemeier G, Leidner R, Bell RB, Weinberg AD
Nature communications 2018 Jul 13;9(1):2724
Nature communications 2018 Jul 13;9(1):2724
Phase I trial of plerixafor combined with decitabine in newly diagnosed older patients with acute myeloid leukemia.
Roboz GJ, Ritchie EK, Dault Y, Lam L, Marshall DC, Cruz NM, Hsu HC, Hassane DC, Christos PJ, Ippoliti C, Scandura JM, Guzman ML
Haematologica 2018 Aug;103(8):1308-1316
Haematologica 2018 Aug;103(8):1308-1316
PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells.
Stecher C, Battin C, Leitner J, Zettl M, Grabmeier-Pfistershammer K, Höller C, Zlabinger GJ, Steinberger P
Frontiers in immunology 2017;8:572
Frontiers in immunology 2017;8:572
Synergistic effect of IL-12 and IL-18 induces TIM3 regulation of γδ T cell function and decreases the risk of clinical malaria in children living in Papua New Guinea.
Schofield L, Ioannidis LJ, Karl S, Robinson LJ, Tan QY, Poole DP, Betuela I, Hill DL, Siba PM, Hansen DS, Mueller I, Eriksson EM
BMC medicine 2017 Jun 15;15(1):114
BMC medicine 2017 Jun 15;15(1):114
TCR-ligand dissociation rate is a robust and stable biomarker of CD8+ T cell potency.
Allard M, Couturaud B, Carretero-Iglesia L, Duong MN, Schmidt J, Monnot GC, Romero P, Speiser DE, Hebeisen M, Rufer N
JCI insight 2017 Jul 20;2(14)
JCI insight 2017 Jul 20;2(14)
Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE.
Zhao D, Guo M, Liu B, Lin Q, Xie T, Zhang Q, Jia X, Shu Q, Liang X, Gao L, Ma C
Journal of leukocyte biology 2017 Dec;102(6):1313-1322
Journal of leukocyte biology 2017 Dec;102(6):1313-1322
Dendritic cell vaccination in combination with docetaxel for patients with metastatic castration-resistant prostate cancer: A randomized phase II study.
Kongsted P, Borch TH, Ellebaek E, Iversen TZ, Andersen R, Met Ö, Hansen M, Lindberg H, Sengeløv L, Svane IM
Cytotherapy 2017 Apr;19(4):500-513
Cytotherapy 2017 Apr;19(4):500-513
BCG vaccination induces HIV target cell activation in HIV-exposed infants in a randomized trial.
Gasper MA, Hesseling AC, Mohar I, Myer L, Azenkot T, Passmore JS, Hanekom W, Cotton MF, Crispe IN, Sodora DL, Jaspan HB
JCI insight 2017 Apr 6;2(7):e91963
JCI insight 2017 Apr 6;2(7):e91963
Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9.
Schwartz JA, Clayton KL, Mujib S, Zhang H, Rahman AK, Liu J, Yue FY, Benko E, Kovacs C, Ostrowski MA
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 15;198(8):3181-3194
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 15;198(8):3181-3194
CD3(bright)CD56(+) T cells associate with pegylated interferon-alpha treatment nonresponse in chronic hepatitis B patients.
Guo C, Shen X, Fu B, Liu Y, Chen Y, Ni F, Ye Y, Sun R, Li J, Tian Z, Wei H
Scientific reports 2016 May 13;6:25567
Scientific reports 2016 May 13;6:25567
CD62L+ NKT cells have prolonged persistence and antitumor activity in vivo.
Tian G, Courtney AN, Jena B, Heczey A, Liu D, Marinova E, Guo L, Xu X, Torikai H, Mo Q, Dotti G, Cooper LJ, Metelitsa LS
The Journal of clinical investigation 2016 Jun 1;126(6):2341-55
The Journal of clinical investigation 2016 Jun 1;126(6):2341-55
Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition.
Cherkassky L, Morello A, Villena-Vargas J, Feng Y, Dimitrov DS, Jones DR, Sadelain M, Adusumilli PS
The Journal of clinical investigation 2016 Aug 1;126(8):3130-44
The Journal of clinical investigation 2016 Aug 1;126(8):3130-44
Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection.
Frederiksen J, Buggert M, Noyan K, Nowak P, Sönnerborg A, Lund O, Karlsson AC
PloS one 2015;10(9):e0137635
PloS one 2015;10(9):e0137635
CD127 expression, exhaustion status and antigen specific proliferation predict sustained virologic response to IFN in HCV/HIV co-infected individuals.
Kared H, Saeed S, Klein MB, Shoukry NH
PloS one 2014;9(7):e101441
PloS one 2014;9(7):e101441
Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy.
Meggyes M, Miko E, Polgar B, Bogar B, Farkas B, Illes Z, Szereday L
PloS one 2014;9(3):e92371
PloS one 2014;9(3):e92371
Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection.
Lanteri MC, Diamond MS, Law JP, Chew GM, Wu S, Inglis HC, Wong D, Busch MP, Norris PJ, Ndhlovu LC
PloS one 2014;9(3):e92134
PloS one 2014;9(3):e92134
Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
Herath S, Benlahrech A, Papagatsias T, Athanasopoulos T, Bouzeboudjen Z, Hervouet C, Klavinskis L, Meiser A, Kelleher P, Dickson G, Patterson S
PloS one 2014;9(2):e88327
PloS one 2014;9(2):e88327
T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
Clayton KL, Haaland MS, Douglas-Vail MB, Mujib S, Chew GM, Ndhlovu LC, Ostrowski MA
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):782-91
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):782-91
The immunomodulatory effects of bevacizumab on systemic immunity in patients with metastatic melanoma.
Mansfield AS, Nevala WK, Lieser EA, Leontovich AA, Markovic SN
Oncoimmunology 2013 May 1;2(5):e24436
Oncoimmunology 2013 May 1;2(5):e24436
Age-related expansion of Tim-3 expressing T cells in vertically HIV-1 infected children.
Tandon R, Giret MT, Sengupta D, York VA, Wiznia AA, Rosenberg MG, Kallas EG, Ndhlovu LC, Nixon DF
PloS one 2012;7(9):e45733
PloS one 2012;7(9):e45733
Tim-3 negatively regulates cytotoxicity in exhausted CD8+ T cells in HIV infection.
Sakhdari A, Mujib S, Vali B, Yue FY, MacParland S, Clayton K, Jones RB, Liu J, Lee EY, Benko E, Kovacs C, Gommerman J, Kaul R, Ostrowski MA
PloS one 2012;7(7):e40146
PloS one 2012;7(7):e40146
Extended co-expression of inhibitory receptors by human CD8 T-cells depending on differentiation, antigen-specificity and anatomical localization.
Baitsch L, Legat A, Barba L, Fuertes Marraco SA, Rivals JP, Baumgaertner P, Christiansen-Jucht C, Bouzourene H, Rimoldi D, Pircher H, Rufer N, Matter M, Michielin O, Speiser DE
PloS one 2012;7(2):e30852
PloS one 2012;7(2):e30852
Upregulation of the Tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
Nebbia G, Peppa D, Schurich A, Khanna P, Singh HD, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, ChinAleong J, Kennedy P, Maini MK
PloS one 2012;7(10):e47648
PloS one 2012;7(10):e47648
Antigen-independent induction of Tim-3 expression on human T cells by the common γ-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway.
Mujib S, Jones RB, Lo C, Aidarus N, Clayton K, Sakhdari A, Benko E, Kovacs C, Ostrowski MA
Journal of immunology (Baltimore, Md. : 1950) 2012 Apr 15;188(8):3745-56
Journal of immunology (Baltimore, Md. : 1950) 2012 Apr 15;188(8):3745-56
Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis.
Idali F, Wahlström J, Dahlberg B, Khademi M, Olsson T, Eklund A, Grunewald J
Respiratory research 2009 May 29;10:42
Respiratory research 2009 May 29;10:42
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TIM-3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM-3 PE-conjugated Monoclonal Antibody (Catalog # FAB2365P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
