PA5-89785

antibody from Invitrogen Antibodies

Targeting: TMEM25 FLJ14399

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89785

Provider

Invitrogen Antibodies

Product name

TMEM25 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: APGPSRHPSL ISSDSNNLKL NNVRLPRENM SLPSNLQLND LTPDSRAVKP ADRQMAQNNS RPELLDPEPG GLLTSQGFIR LPVLGYIYRV SSVSSDEIWL; Positive Samples: U-87MG, OVCAR3, LO2, Mouse brain, Mouse kidney, Rat brain, Rat kidney; Cellular Location: Cell membrane, Single-pass type I membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.09 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using TMEM25 Polyclonal antibody (Product # PA5-89785) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TMEM25 in extracts of various cell lines using TMEM25 Polyclonal Antibody (Product # PA5-89785) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TMEM25 was performed in L929 cells using TMEM25 Polyclonal Antibody (Product # PA5-89785) at a dilution of 1:100. Blue: DAPI for nuclear staining.