Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30117 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCCA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PCCA Polyclonal Antibody (Product # PA5-30117). Sample (30 µg of whole cell lysate). A: HeLa . 7.5% SDS PAGE. PCCA Polyclonal Antibody (Product # PA5-30117) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Kidney (Lane 1), Mouse Liver (Lane 2), Mouse Spleen (Lane 3), Mouse Ovary (Lane 4) and Rat Ovary (Lane 5). The blot was probed with Anti-PCCA Polyclonal Antibody (Product # PA5-30117, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 77 kDa band corresponding to PCCA was observed across the tissues tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PCCA (Product # PA5-30117) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.