TA504639

antibody from OriGene

Targeting: CYB5R1 humb5R2, NQO3A2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504639

Provider

OriGene

Proper citation

OriGene Cat#TA504639, RRID:AB_2622495

Product name

CYB5R1 mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)

Antibody type

Monoclonal

Description

CYB5R1 mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

CYB5R1

Isotype

IgG

Antibody clone number

OTI2D10

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CYB5R1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R1 (RC205833, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R1.

Validation comment

WB