NBP1-84449

antibody from Novus Biologicals

Targeting: PLA2R1 CLEC13C, PLA2-R, PLA2G1R, PLA2IR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-84449

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-84449, RRID:AB_11028306

Product name

Rabbit Polyclonal PLA2R1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human PLA2R1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Prevalence of Enhanced Granular Expression of Thrombospondin Type-1 Domain-Containing 7A in the Glomeruli of Japanese Patients with Idiopathic Membranous Nephropathy.

Iwakura T, Ohashi N, Kato A, Baba S, Yasuda H
PloS one 2015;10(9):e0138841

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Membranous Nephropathy Associated With Immunological Disorder-Related Liver Disease: A Retrospective Study of 10 Cases.

Dauvergne M, Moktefi A, Rabant M, Vigneau C, Kofman T, Burtey S, Corpechot C, Stehlé T, Desvaux D, Rioux-Leclercq N, Rouvier P, Knebelmann B, Boffa JJ, Frouget T, Daugas E, Jablonski M, Dahan K, Brocheriou I, Remy P, Grimbert P, Lang P, Chazouilleres O, Sahali D, Audard V
Medicine 2015 Jul;94(30):e1243

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Prevalence of anti-phospholipase A2 receptor antibodies in Japanese patients with membranous nephropathy.

Akiyama S, Akiyama M, Imai E, Ozaki T, Matsuo S, Maruyama S
Clinical and experimental nephrology 2015 Aug;19(4):653-60

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Kidney biopsy is a sensitive tool for retrospective diagnosis of PLA2R-related membranous nephropathy.

Svobodova B, Honsova E, Ronco P, Tesar V, Debiec H
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2013 Jul;28(7):1839-44

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PLA2R1 kills cancer cells by inducing mitochondrial stress.

Augert A, Vindrieux D, Girard CA, Le Calvé B, Gras B, Ferrand M, Bouchet BP, Puisieux A, de Launoit Y, Simonnet H, Lambeau G, Bernard D
Free radical biology & medicine 2013 Dec;65:969-977

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Enhanced expression of the M-type phospholipase A2 receptor in glomeruli correlates with serum receptor antibodies in primary membranous nephropathy.

Hoxha E, Kneißler U, Stege G, Zahner G, Thiele I, Panzer U, Harendza S, Helmchen UM, Stahl RA
Kidney international 2012 Oct;82(7):797-804

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Autoantibodies specific for the phospholipase A2 receptor in recurrent and De Novo membranous nephropathy.

Debiec H, Martin L, Jouanneau C, Dautin G, Mesnard L, Rondeau E, Mousson C, Ronco P
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2011 Oct;11(10):2144-52

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PLA2R autoantibodies and PLA2R glomerular deposits in membranous nephropathy.

Debiec H, Ronco P
The New England journal of medicine 2011 Feb 17;364(7):689-90

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLA2R1 Antibody [NBP1-84449] - Analysis in human kidney tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PLA2R1 Antibody [NBP1-84449] - Analysis in control (vector only transfected HEK293T lysate) and PLA2R1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining of human kidney shows weak membranous positivity in cells in glomeruli.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining in human kidney and pancreas tissues . Corresponding PLA2R1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining o human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.