M06243

antibody from Boster Biological Technology

Targeting: H1-2 H1.2, H1c, H1F2, H1s-1, HIST1H1C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

M06243

Provider

Boster Biological Technology

Product name

Anti-Histone H1.2 Monoclonal Antibody

Antibody type

Monoclonal

Description

Rabbit IgG Monoclonal antibody for Histone H1.2 detection. Tested positive for WB, IHC, IF, ICC, ChIP in Human, Mouse, Rat

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

AEDF-8

Vial size

100μg

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of HIST1H1C using anti-HIST1H1C antibody (M06243) in (1) MCF7 cell lysate (2) NIH/3T3 cell lysate; (3) C6 cell lysate.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H1C antigen affinity purified polyclonal antibody (Catalog # M06243) at 0.5 ug/mL overnight at 4

Additional image