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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-24714 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH6A1 Monoclonal Antibody (147CT8.3.4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 147CT8.3.4
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH6A1 in MCF-7 lysate using 20 µg of sample per lane. The membrane was probed with ALDH6A1 monoclonal antibody (Product # MA5-24714) with a diluion of 1:1000 and a goat anti-mouse IgG H&L(HRP) secondary antibody with a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH6A1 in MCF-7 lysate using 20 µg of sample per lane. The membrane was probed with ALDH6A1 monoclonal antibody (Product # MA5-24714) with a diluion of 1:1000 and a goat anti-mouse IgG H&L(HRP) secondary antibody with a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ALDH6A1 in MCF-7 cells. Cells were probed with a ALDH6A1 monoclonal antibody (Product # MA5-24714) using a dilution of 1:25, followed by Alexa Fluor 488-conjugated goat anti-mouse lgG, DAPI and Alexa Fluor 555 conjugated with Phalloidin using a dilution of 1:400.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human colon tissue. Sample was probed with a ALDH6A1 monoclonal antibody (Product # MA5-24714) with a dilution of 1:25. Detection was performed with peroxidase-conjugated goat anti-mouse IgG and DAB using a dilution of 1:400.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human liver tissue. Sample was probed with a ALDH6A1 monoclonal antibody (Product # MA5-24714) with a dilution of 1:25. Detection was performed with peroxidase-conjugated goat anti-mouse IgG and DAB using a dilution of 1:400.
