NBP2-59781

antibody from Novus Biologicals

Targeting: PPIB B, CYP-S1, CYPB, OI9, PPIase, SCYLP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-59781

Provider

Novus Biologicals

Product name

Mouse Monoclonal Cyclophilin B Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human, mouse, rat Cyclophilin B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cyclophilin B Antibody (5479) [NBP2-59781] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cyclophilin B Antibody (5479) [NBP2-59781] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PPIB monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cyclophilin B Antibody (5479) [NBP2-59781] - Human placenta shows strong cytoplasmic immunoreactivity in trophoblasts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cyclophilin B Antibody (5479) [NBP2-59781] - Human prostate shows cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cyclophilin B Antibody (5479) [NBP2-59781] - Human testis shows strong cytoplasmic immunoreactivity in a subset of cells in seminiferous tubules.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cyclophilin B Antibody (5479) [NBP2-59781] - Human stomach shows cytoplasmic positivity in glandular and lymphoid cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cyclophilin B Antibody (5479) [NBP2-59781] - Human cerebral cortex shows cytoplasmic immunoreactivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cyclophilin B Antibody (5479) [NBP2-59781] - Human pancreas shows strong cytoplasmic positivity in the exocrine cells and weak immunoreactivity in the endocrine cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cyclophilin B Antibody (5479) [NBP2-59781] - Human skeletal muscle shows very weak cytoplasmic positivity in muscle fibers.