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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- PA5-54963 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SKAP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LPEEIRNLLA DVETFVADIL KGENLSKKAK EKRESLIKKI KDVKSIYLQE FQDKGDAEDG EEYDDPFAGP PDTISLASER YDKDDEAPSD GAQFPPIAAQ DLPFVLKAGY LEKRRKDHSF LGFEWQKRWC ALSKTVFYY
- Concentration
- 0.23 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SKAP2 in control (vector only transfected HEK293T lysate) and SKAP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SKAP2 Polyclonal Antibody (Product # PA5-54963).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SKAP2 in human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a SKAP2 Polyclonal Antibody (Product # PA5-54963).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SKAP2 in human colon using SKAP2 Polyclonal Antibody (Product # PA5-54963).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SKAP2 in human tonsil using SKAP2 Polyclonal Antibody (Product # PA5-54963).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SKAP2 in human lymph node shows distinct cytoplasmic positivity in a subset of cells. Samples were probed using a SKAP2 Polyclonal Antibody (Product # PA5-54963).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SKAP2 in human lymph node and pancreas tissues using SKAP2 Polyclonal Antibody (Product # PA5-54963). Corresponding SKAP2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SKAP2 in human colon, lymph node, pancreas and tonsil using SKAP2 Polyclonal Antibody (Product # PA5-54963) (A) shows similar protein distribution across tissues to an independent SKAP2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SKAP2 in human pancreas using SKAP2 Polyclonal Antibody (Product # PA5-54963) shows low expression as expected.
