PA5-111756

antibody from Invitrogen Antibodies

Targeting: STIM1 D11S4896E, GOK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Recommended by provider)

Antibody Data

Product number

PA5-111756

Provider

Invitrogen Antibodies

Product name

STIM1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.85 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of STIM1 was performed in RBL (lanes 1 and 4), HL-60 (lanes 2 and 5), and Jurkat (lanes 3 and 6) cell lysates. Lane 1,2,3: STIM1 (extracellular) Antibody (Product # PA5-111756) at a dilution of 1:1000. Lane 4,5,6: STIM1 (extracellular) Antibody preincubated with the negative control antigen.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of STIM1 was performed in RBL (lanes 1 and 4), HL-60 (lanes 2 and 5), and Jurkat (lanes 3 and 6) cell lysates. Lane 1,2,3: STIM1 (extracellular) Antibody (Product # PA5-111756) at a dilution of 1:1000. Lane 4,5,6: STIM1 (extracellular) Antibody preincubated with the negative control antigen.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of STIM1 in live RBL cells. A) Extracellular staining of cells with STIM1 (extracellular) Antibody (Product # PA5-111756) (1:50) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B) Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C) Merged image of panels A and B.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of STIM1 in paraffin-embedded rat stomach tissue sections using STIM1 (extracellular) Antibody (Product # PA5-111756) at a dilution of 1:100. STIM1 is expressed in the parietal cells of the gastric mucosa (arrows). Hematoxilin is used as the counterstain.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of STIM1 in intact RBL (A) and Jurkat (B) cells. Black: Cells and FITC-conjugated goat anti-rabbit antibody. Green: Cells and STIM1 (extracellular) Antibody (Product # PA5-111756), (5-10 µg antibody/0.5-1x10^6 cells) and FITC-conjugated goat anti-rabbit antibody.