NBP2-59382

antibody from Novus Biologicals

Targeting: SCNN1A ENaCalpha, SCNN1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-59382

Provider

Novus Biologicals

Product name

Mouse Monoclonal epithelial Sodium Channel alpha Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects 85kDa.

Reactivity

Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Storage

Store at -20C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salf diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.