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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF3720 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse RGM-C/Hemojuvelin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse RGM-C in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) RGM-B is observed and less than 5% cross-reactivity with rhRGM-A is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q6ZVN8- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Functional diversity of TMPRSS6 isoforms and variants expressed in hepatocellular carcinoma cell lines.
Transcriptome analysis reveals TMPRSS6 isoforms with distinct functionalities.
Downregulation of hemojuvelin prevents inhibitory effects of bone morphogenetic proteins on iron metabolism in hepatocellular carcinoma.
Dion SP, Béliveau F, Morency LP, Désilets A, Najmanovich R, Leduc R
Scientific reports 2018 Aug 22;8(1):12562
Scientific reports 2018 Aug 22;8(1):12562
Transcriptome analysis reveals TMPRSS6 isoforms with distinct functionalities.
Dion SP, Béliveau F, Désilets A, Ghinet MG, Leduc R
Journal of cellular and molecular medicine 2018 Apr;22(4):2498-2509
Journal of cellular and molecular medicine 2018 Apr;22(4):2498-2509
Downregulation of hemojuvelin prevents inhibitory effects of bone morphogenetic proteins on iron metabolism in hepatocellular carcinoma.
Maegdefrau U, Arndt S, Kivorski G, Hellerbrand C, Bosserhoff AK
Laboratory investigation; a journal of technical methods and pathology 2011 Nov;91(11):1615-23
Laboratory investigation; a journal of technical methods and pathology 2011 Nov;91(11):1615-23
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- RGM-C/Hemojuvelin in HepG2 Human Cell Line. RGM-C/Hemojuvelin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse RGM-C/Hemojuvelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3720) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
