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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB1172F - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal ALCAM/CD166 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse ALCAM in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ALCAM is observed, and less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM-1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Heterogeneity and dynamics of active Kras-induced dysplastic lineages from mouse corpus stomach.
Min J, Vega PN, Engevik AC, Williams JA, Yang Q, Patterson LM, Simmons AJ, Bliton RJ, Betts JW, Lau KS, Magness ST, Goldenring JR, Choi E
Nature communications 2019 Dec 5;10(1):5549
Nature communications 2019 Dec 5;10(1):5549
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of ALCAM/CD166 in Mouse Splenocytes by Flow Cytometry. Mouse Splenocytes either (A) activated or (B) resting were stained with Goat Anti-Mouse ALCAM/CD166 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1172F) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # IC108F). View our protocol for Staining Membrane-associated Proteins.
