FAB6561P

antibody from Novus Biologicals

Targeting: ALCAM CD166, MEMD

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

FAB6561P

Provider

Novus Biologicals

Product name

Mouse Monoclonal ALCAM/CD166 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human ALCAM in Western blots. Shows approximately 50% cross-reactivity with recombinant mouse OCAM and no cross-reactivity with recombinant human (rh) BCAM, rhEpCAM, rhMCAM, or rhNCAM-L1.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium.

Mount S, Kanda P, Parent S, Khan S, Michie C, Davila L, Chan V, Davies RA, Haddad H, Courtman D, Stewart DJ, Davis DR
Stem cell research & therapy 2019 Nov 4;10(1):316

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Methionine is a metabolic dependency of tumor-initiating cells.

Wang Z, Yip LY, Lee JHJ, Wu Z, Chew HY, Chong PKW, Teo CC, Ang HY, Peh KLE, Yuan J, Ma S, Choo LSK, Basri N, Jiang X, Yu Q, Hillmer AM, Lim WT, Lim TKH, Takano A, Tan EH, Tan DSW, Ho YS, Lim B, Tam WL
Nature medicine 2019 May;25(5):825-837

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Reduced CD146 expression promotes tumorigenesis and cancer stemness in colorectal cancer through activating Wnt/β-catenin signaling.

Liu D, Du L, Chen D, Ye Z, Duan H, Tu T, Feng J, Yang Y, Chen Q, Yan X
Oncotarget 2016 Jun 28;7(26):40704-40718

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Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas.

Bogachek MV, Park JM, De Andrade JP, Lorenzen AW, Kulak MV, White JR, Gu VW, Wu VT, Weigel RJ
Stem cell reports 2016 Dec 13;7(6):1140-1151

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CD166/ALCAM expression is characteristic of tumorigenicity and invasive and migratory activities of pancreatic cancer cells.

Fujiwara K, Ohuchida K, Sada M, Horioka K, Ulrich CD 3rd, Shindo K, Ohtsuka T, Takahata S, Mizumoto K, Oda Y, Tanaka M
PloS one 2014;9(9):e107247

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Establishment of highly tumorigenic human colorectal cancer cell line (CR4) with properties of putative cancer stem cells.

Rowehl RA, Burke S, Bialkowska AB, Pettet DW 3rd, Rowehl L, Li E, Antoniou E, Zhang Y, Bergamaschi R, Shroyer KR, Ojima I, Botchkina GI
PloS one 2014;9(6):e99091

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Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.

Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Höller C, Wacheck V, Pehamberger H, Valent P
PloS one 2014;9(1):e84417

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Activated leukocyte cell adhesion molecule (CD166): an "inert" cancer stem cell marker for non-small cell lung cancer?

Tachezy M, Zander H, Wolters-Eisfeld G, Müller J, Wicklein D, Gebauer F, Izbicki JR, Bockhorn M
Stem cells (Dayton, Ohio) 2014 Jun;32(6):1429-36

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Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.

Albert R, Veréb Z, Csomós K, Moe MC, Johnsen EO, Olstad OK, Nicolaissen B, Rajnavölgyi E, Fésüs L, Berta A, Petrovski G
PloS one 2012;7(10):e47187

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CD166(pos) subpopulation from differentiated human ES and iPS cells support repair of acute lung injury.

Soh BS, Zheng D, Li Yeo JS, Yang HH, Ng SY, Wong LH, Zhang W, Li P, Nichane M, Asmat A, Wong PS, Wong PC, Su LL, Mantalaris SA, Lu J, Xian W, McKeon F, Chen J, Lim EH, Lim B
Molecular therapy : the journal of the American Society of Gene Therapy 2012 Dec;20(12):2335-46

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Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.

Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
PloS one 2011;6(5):e19988

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Epstein-Barr virus latent membrane protein 1 induces cancer stem/progenitor-like cells in nasopharyngeal epithelial cell lines.

Kondo S, Wakisaka N, Muramatsu M, Zen Y, Endo K, Murono S, Sugimoto H, Yamaoka S, Pagano JS, Yoshizaki T
Journal of virology 2011 Nov;85(21):11255-64

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Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension.

Firth AL, Yao W, Ogawa A, Madani MM, Lin GY, Yuan JX
American journal of physiology. Cell physiology 2010 May;298(5):C1217-25

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of ALCAM/CD166 in Human Whole Blood Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Mouse Anti-Human ALCAM/CD166 PE-conjugated Monoclonal Antibody (Catalog # FAB6561P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.