LF-MA0207

antibody from Invitrogen Antibodies

Targeting: BID

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LF-MA0207

Provider

Invitrogen Antibodies

Product name

Anti-BID Monoclonal Antibody (8D2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is A431 cells.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

8D2

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-BID Monoclonal Antibody (8D2) (Product # LF-MA0207) and a 20 kDa band corresponding to BID was observed across cell lines and tissue extracts tested and decreased upon Staurosporine and also with Actinomycin D along with TNF Alpha treatment. The expression of BID is reported to be low in Mouse Brain compared to Mouse Spleen and Mouse Thymus. Fig.a shows whole cell extracts (30 µg lysate) of A-431 (Lane 1), A-431 treated with Staurosporine (1uM for 4 hr) (Lane 2), A-431 treated with Staurosporine (1uM for 8 hr) (Lane 3) and A-431 treated with Actinomycin along with TNF Alpha (5ug/ml and 10ng/ml for 5 hr) (Lane 4), Fig.b shows whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Jurkat (Lane 2), MOLT-4 (Lane 3), HeLa (Lane 4), PC-3 (Lane 5), SH-SY5Y (Lane 6), tissue extracts of Mouse Spleen (Lane 7), Mouse Thymus (Lane 8) and Mouse Brain (Lane 9) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 ug/ml) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent.