Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-56107 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56107, RRID:AB_2065642
- Product name
- Rabbit Polyclonal BID Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by capspase-8 following death receptor activation. The term "Bid" was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Krajewska M, Rosenthal RE, Mikolajczyk J, Stennicke HR, Wiesenthal T, Mai J, Naito M, Salvesen GS, Reed JC, Fiskum G, Krajewski S
Experimental neurology 2004 Oct;189(2):261-79
Experimental neurology 2004 Oct;189(2):261-79
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bid Antibody [NB100-56107] - Formalin-fixed, paraffin-embedded human gastric carcinoma tissue array stained for Bid expression using this antibody at 1:2000. Hematoxylin-eosin counterstain. Variable Bid expression is seen between patient samples.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bid Antibody [NB100-56107] - Six representative cores from a human gastric carcinoma formalin-fixed, paraffin-embedded tissue microarray stained for Bid expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.