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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Other assay [2]
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- Product number
- MA5-15705 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PERK Monoclonal Antibody (5G5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15705 targets EIF2AK3 in WB applications and shows reactivity with Human samples.
- Antibody clone number
- 5G5
- Concentration
- Conc. Not Determined
Submitted references Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.
Combined effects of Lenvatinib and iodine-131 on cell apoptosis in nasopharyngeal carcinoma through inducing endoplasmic reticulum stress.
Ullah M, Liu DD, Rai S, Razavi M, Concepcion W, Thakor AS
Stem cell research & therapy 2020 Sep 14;11(1):398
Stem cell research & therapy 2020 Sep 14;11(1):398
Combined effects of Lenvatinib and iodine-131 on cell apoptosis in nasopharyngeal carcinoma through inducing endoplasmic reticulum stress.
Wang G, Zhuang J, Ni J, Ye Y, He S, Xia W
Experimental and therapeutic medicine 2018 Oct;16(4):3325-3332
Experimental and therapeutic medicine 2018 Oct;16(4):3325-3332
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF2AK3 using EIF2AK3 monoclonal antibody (Product # MA5-15705) in HEK293 (1) and EIF2AK3 (AA: 929-1116) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF2AK3 using EIF2AK3 monoclonal antibody (Product # MA5-15705) in HEK293 (1) and EIF2AK3 (AA: 929-1116) human IgG Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 Signaling pathway analysis. a Western blot and protein quantification showing the expression of p-ERK, ERK, p-MEK, MEK, and beta-actin in kidney tissue. b Western blot and protein quantification showing the expression of p-Akt, Akt, and beta-actin in kidney tissue. c Western blot and protein quantification showing the expression of SIRT3, eNOS, and beta-actin in kidney tissue. Each group has n = 3 mice. Significant difference a p < 0.05: relative to untreated control; b p < 0.05: relative to AKI; c p < 0.05: relative to AKI-EV; d p < 0.05: relative to AKI-EV-pFUS
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Combination of LEB and I-131 regulates apoptosis of nasopharyngeal carcinoma cells through ER stress. (A) ATF6, IER1, RERK and CHOP expression levels in HK-1 cells following treatment with LEB or/and I-131 determined by western blotting. (B) Analysis of mRNA expression levels of ATF6, IER1, RERK and CHOP expression levels in HK-1 cells. (C) Effects of LEB or/and I-131 on ER in HK-1 cells (magnification, x100). (D) GRP78 expression in HK-1 cells following combined treatment of LEB and I-131 determined by immunofluorescence (magnification, x20). (E) Adhered morphology of HK-1 cells following treatment with LEB or/and I-131 (magnification, x20). (F) JNK and P38 expression levels in HK-1 following treatment with LEB or/and I-131. The data are presented as the mean + standard error of the mean of three independent experiments. **P
