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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- NBP2-33857 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Anillin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Anillin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Systematic antibody generation and validation via tissue microarray technology leading to identification of a novel protein prognostic panel in breast cancer.
Generation of monospecific antibodies based on affinity capture of polyclonal antibodies.
O Leary PC, Penny SA, Dolan RT, Kelly CM, Madden SF, Rexhepaj E, Brennan DJ, McCann AH, Pontén F, Uhlén M, Zagozdzon R, Duffy MJ, Kell MR, Jirström K, Gallagher WM
BMC cancer 2013 Apr 2;13:175
BMC cancer 2013 Apr 2;13:175
Generation of monospecific antibodies based on affinity capture of polyclonal antibodies.
Hjelm B, Forsström B, Igel U, Johannesson H, Stadler C, Lundberg E, Ponten F, Sjöberg A, Rockberg J, Schwenk JM, Nilsson P, Johansson C, Uhlén M
Protein science : a publication of the Protein Society 2011 Nov;20(11):1824-35
Protein science : a publication of the Protein Society 2011 Nov;20(11):1824-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Anillin Antibody [NBP2-33857] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ANLN antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-33857] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-33857] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-33857] - Staining in human testis and skeletal muscle tissues using anti-ANLN antibody. Corresponding ANLN RNA-seq data are presented for the same tissues.
