PA5-78003

antibody from Invitrogen Antibodies

Targeting: HLA-DQB1 CELIAC1, HLA-DQB, IDDM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78003

Provider

Invitrogen Antibodies

Product name

HLA-DQB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Raji

Concentration

0.7 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HLA DQB1 in Jurkat whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with HLA DQB1 polyclonal antibody (Product # PA5-78003) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HLA DQB1 antibody detects HLA DQB1 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract.12% SDS-PAGE. HLA DQB1 antibody HLA-DQB1 Polyclonal Antibody (Product # PA5-78003) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HLA DQB1 antibody detects HLA DQB1 protein at cell membrane and endoplasmic reticulum by immunofluorescent analysis. Sample: Raji cells were fixed in iced-cold MeOH for 5 min. Green: HLA DQB1 protein stained by HLA DQB1 antibody (Product # PA5-78003) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HLA DQB1 antibody detects HLA DQB1 protein at cell membrane and endoplasmic reticulum by immunofluorescent analysis. Sample: Raji cells were fixed in iced-cold MeOH for 5 min. Green: HLA DQB1 protein stained by HLA DQB1 antibody (Product # PA5-78003) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HLA DQB1 antibody detects HLA DQB1 protein at cytoplasm and membrane on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. HLA DQB1 antibody (Product # PA5-78003) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.