TA506798

antibody from OriGene

Targeting: POU5F1 MGC22487, OCT3, Oct4, OTF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA506798

Provider

OriGene

Proper citation

OriGene Cat#TA506798, RRID:AB_2623901

Product name

POU5F1 mouse monoclonal antibody, clone OTI7H1 (formerly 7H1)

Antibody type

Monoclonal

Description

POU5F1 mouse monoclonal antibody, clone OTI7H1 (formerly 7H1)

Host

Mouse

Conjugate

Unconjugated

Epitope

POU5F1

Isotype

IgG

Antibody clone number

OTI7H1

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU5F1 (RC211998, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU5F1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POU5F1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of CRL1973 cells using anti-POU5F1 mouse monoclonal antibody (TA506798).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-POU5F1 mouse monoclonal antibody (TA506798) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POU5F1(RC211998).

Validation comment

IF