Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- FAB5260P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-28R alpha/IFN-lambda R1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-28 R alpha/IFN-lambda R1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-28 alpha is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Hepatitis B Virus e Antigen Activates the Suppressor of Cytokine Signaling 2 to Repress Interferon Action.
Yu Y, Wan P, Cao Y, Zhang W, Chen J, Tan L, Wang Y, Sun Z, Zhang Q, Wan Y, Zhu Y, Liu F, Wu K, Liu Y, Wu J
Scientific reports 2017 May 11;7(1):1729
Scientific reports 2017 May 11;7(1):1729
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IL-28 R alpha/IFN-lambda R1 in PBMCs by Flow Cytometry. Peripheral blood mononuclear cells (PBMCs) either untreated (A) or treated (B) with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight were stained with Mouse Anti-Human IL-28 R alpha/IFN-lambda R1 PE-conjugated Monoclonal Antibody (Catalog # FAB5260P) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IL-28 R alpha/IFN-lambda R1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-28 R alpha/IFN-lambda R1 PE-conjugated Monoclonal Antibody (Catalog # FAB5260P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.