328612

antibody from BioLegend

Targeting: LAMP1 CD107a

Immunocytochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

328612

Provider

BioLegend

Proper citation

BioLegend Cat#328612, RRID:AB_1227506

Product name

Alexa Fluor® 647 anti-human CD107a (LAMP-1) [H4A3]

Antibody type

Monoclonal

Antigen

Human adult adherent peripheral blood cells

Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane gly­coprotein. The mature CD107a is heavily glycosylated from a 40 kD core protein. It is expressed by activated platelets, acti­vated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. This molecule is located on the luminal side of lysosomes and has been suggested to play a role in the protection of lysosomes membrane from lysosomal hydrolases. Upon activation, CD107a is transferred to the cell membrane surface. It plays a role in cell adhesion and regula­tion of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Vial size

100 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.v

References

Submitted references

Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.

Watanabe A, Tatematsu M, Saeki K, Shibata S, Shime H, Yoshimura A, Obuse C, Seya T, Matsumoto M
The Journal of biological chemistry 2011 Mar 25;286(12):10702-11

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Hook2 is involved in the morphogenesis of the primary cilium.

Baron Gaillard CL, Pallesi-Pocachard E, Massey-Harroche D, Richard F, Arsanto JP, Chauvin JP, Lecine P, Krämer H, Borg JP, Le Bivic A
Molecular biology of the cell 2011 Dec;22(23):4549-62

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Thrombin-activated human peripheral blood platelets stained with H4A3 Alexa Fluor® 647

Conjugate

Red dye