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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [4]
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- Product number
- PA1-654A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LAMP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-654A detects Lamp1 from human samples.
- Concentration
- 1 mg/mL
Submitted references Upregulation of ATG7 attenuates motor neuron dysfunction associated with depletion of TARDBP/TDP-43.
Intracellular localization of polymyxins in human alveolar epithelial cells.
Metformin exacerbates and simvastatin attenuates myelin damage in high fat diet-fed C57BL/6 J mice.
The Rab11-binding protein RELCH/KIAA1468 controls intracellular cholesterol distribution.
Human plasma C3 is essential for the development of memory B, but not T, lymphocytes.
PIKfyve activity regulates reformation of terminal storage lysosomes from endolysosomes.
The PKD domain distinguishes the trafficking and amyloidogenic properties of the pigment cell protein PMEL and its homologue GPNMB.
NADPH oxidase NOX2 defines a new antagonistic role for reactive oxygen species and cAMP/PKA in the regulation of insulin secretion.
Retromer disruption promotes amyloidogenic APP processing.
Actin cytoskeleton-dependent Rab GTPase-regulated angiotensin type I receptor lysosomal degradation studied by fluorescence lifetime imaging microscopy.
VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD.
VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD.
Donde A, Sun M, Jeong YH, Wen X, Ling J, Lin S, Braunstein K, Nie S, Wang S, Chen L, Wong PC
Autophagy 2020 Apr;16(4):672-682
Autophagy 2020 Apr;16(4):672-682
Intracellular localization of polymyxins in human alveolar epithelial cells.
Ahmed MU, Velkov T, Zhou QT, Fulcher AJ, Callaghan J, Zhou F, Chan K, Azad MAK, Li J
The Journal of antimicrobial chemotherapy 2019 Jan 1;74(1):48-57
The Journal of antimicrobial chemotherapy 2019 Jan 1;74(1):48-57
Metformin exacerbates and simvastatin attenuates myelin damage in high fat diet-fed C57BL/6 J mice.
Ciric D, Martinovic T, Petricevic S, Trajkovic V, Bumbasirevic V, Kravic-Stevovic T
Neuropathology : official journal of the Japanese Society of Neuropathology 2018 Oct;38(5):468-474
Neuropathology : official journal of the Japanese Society of Neuropathology 2018 Oct;38(5):468-474
The Rab11-binding protein RELCH/KIAA1468 controls intracellular cholesterol distribution.
Sobajima T, Yoshimura SI, Maeda T, Miyata H, Miyoshi E, Harada A
The Journal of cell biology 2018 May 7;217(5):1777-1796
The Journal of cell biology 2018 May 7;217(5):1777-1796
Human plasma C3 is essential for the development of memory B, but not T, lymphocytes.
Jiménez-Reinoso A, Marin AV, Subias M, López-Lera A, Román-Ortiz E, Payne K, Ma CS, Arbore G, Kolev M, Freeley SJ, Kemper C, Tangye SG, Fernández-Malavé E, Rodríguez de Córdoba S, López-Trascasa M, Regueiro JR
The Journal of allergy and clinical immunology 2018 Mar;141(3):1151-1154.e14
The Journal of allergy and clinical immunology 2018 Mar;141(3):1151-1154.e14
PIKfyve activity regulates reformation of terminal storage lysosomes from endolysosomes.
Bissig C, Hurbain I, Raposo G, van Niel G
Traffic (Copenhagen, Denmark) 2017 Nov;18(11):747-757
Traffic (Copenhagen, Denmark) 2017 Nov;18(11):747-757
The PKD domain distinguishes the trafficking and amyloidogenic properties of the pigment cell protein PMEL and its homologue GPNMB.
Theos AC, Watt B, Harper DC, Janczura KJ, Theos SC, Herman KE, Marks MS
Pigment cell & melanoma research 2013 Jul;26(4):470-86
Pigment cell & melanoma research 2013 Jul;26(4):470-86
NADPH oxidase NOX2 defines a new antagonistic role for reactive oxygen species and cAMP/PKA in the regulation of insulin secretion.
Li N, Li B, Brun T, Deffert-Delbouille C, Mahiout Z, Daali Y, Ma XJ, Krause KH, Maechler P
Diabetes 2012 Nov;61(11):2842-50
Diabetes 2012 Nov;61(11):2842-50
Retromer disruption promotes amyloidogenic APP processing.
Sullivan CP, Jay AG, Stack EC, Pakaluk M, Wadlinger E, Fine RE, Wells JM, Morin PJ
Neurobiology of disease 2011 Aug;43(2):338-45
Neurobiology of disease 2011 Aug;43(2):338-45
Actin cytoskeleton-dependent Rab GTPase-regulated angiotensin type I receptor lysosomal degradation studied by fluorescence lifetime imaging microscopy.
Li H, Yu P, Sun Y, Felder RA, Periasamy A, Jose PA
Journal of biomedical optics 2010 Sep-Oct;15(5):056003
Journal of biomedical optics 2010 Sep-Oct;15(5):056003
VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD.
Tresse E, Salomons FA, Vesa J, Bott LC, Kimonis V, Yao TP, Dantuma NP, Taylor JP
Autophagy 2010 Feb;6(2):217-27
Autophagy 2010 Feb;6(2):217-27
VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD.
Tresse E, Salomons FA, Vesa J, Bott LC, Kimonis V, Yao TP, Dantuma NP, Taylor JP
Autophagy 2010 Feb;6(2):217-27
Autophagy 2010 Feb;6(2):217-27
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LAMP1 was performed by loading 20 µg of HeLa cell lysate and 5 µl of Molecular Weight Protein Ladder per well onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an anti-LAMP1 polyclonal antibody (Product # PA1-654A) at a dilution of 1:500 for one hour at room temperature, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 32260) at a dilution of 1:100,000 for 1hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Lamp1 (red) in HeLa cells using Product # PA1-654A.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Representative confocal lazer microscopic images of double labeled immunofluorescence-stained sciatic nerve sections for LC3 (green) and LAMP1 (red). White arrows indicate yellow colocalization of LC3 and LAMP1.
