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- References [3]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- ABIN1449615 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Lysosomal-Associated Membrane Protein 1 (LAMP1) (N-Term), (AA 131-161) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 131~161 amino acids from the N-terminal region of Human LAMP1
- Description
- Protein A column, followed by peptide affinity purification. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- N-Term,AA 131-161
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras.
The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes.
Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences.
Byun JY, Yoon CH, An S, Park IC, Kang CM, Kim MJ, Lee SJ
Carcinogenesis 2009 Nov;30(11):1880-8
Carcinogenesis 2009 Nov;30(11):1880-8
The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes.
Sawada R, Jardine KA, Fukuda M
The Journal of biological chemistry 1993 Apr 25;268(12):9014-22
The Journal of biological chemistry 1993 Apr 25;268(12):9014-22
Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences.
Fukuda M, Viitala J, Matteson J, Carlsson SR
The Journal of biological chemistry 1988 Dec 15;263(35):18920-8
The Journal of biological chemistry 1988 Dec 15;263(35):18920-8
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of CD107a / LAMP1 Antibody (N-term) Cat.-No AP32183PU-N in Y79 cell line lysates (35ug/lane). LAMP1 (arrow) was detected using the purified Pab (1:60 dilution).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of CD107a / LAMP1 Antibody (N-term) Cat.-No AP32183PU-N in mouse lung tissue lysates (35ug/lane). LAMP1 (arrow) was detected using the purified Pab
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD107a / LAMP1 Antibody (N-term) Cat.-No AP32183PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
