Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-16371 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 18 Monoclonal Antibody (SP69)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is prostate or breast adenocarcinoma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP69
- Vial size
- 500 µL
- Concentration
- 0.204 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of A431 Cells using anti-Keratin 18 Monoclonal Antibody (Product # MA5-16371). The recommened dilution for this antibody in western blot applications is 1:25.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Keratin 18 using anti-Keratin 18 Monoclonal Antibody (Product # MA5-16371) in Breast Adenocarcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:200.