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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF3646 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DNER Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human DNER in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse DNER is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8NFT8- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The Notch ligand DNER regulates macrophage IFNγ release in chronic obstructive pulmonary disease.
Ballester-López C, Conlon TM, Ertüz Z, Greiffo FR, Irmler M, Verleden SE, Beckers J, Fernandez IE, Eickelberg O, Yildirim AÖ
EBioMedicine 2019 May;43:562-575
EBioMedicine 2019 May;43:562-575
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human DNER by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3646) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
