M2TNFAI

antibody from Invitrogen Antibodies

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

M2TNFAI

Provider

Invitrogen Antibodies

Product name

TNF alpha Monoclonal Antibody (E10042)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

M2TNFAI targets TNF Alpha in ELISA applications and shows reactivity with Human samples.

Antibody clone number

E10042

Concentration

1 mg/mL

References

Submitted references

Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.

Montenegro SM, Abath FG, Domingues AL, Melo WG, Morais CN, Coutinho EM, Mahanty S, Wynn TA
Infection and immunity 2002 Nov;70(11):5903-12

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Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.

Montenegro SM, Abath FG, Domingues AL, Melo WG, Morais CN, Coutinho EM, Mahanty S, Wynn TA
Infection and immunity 2002 Nov;70(11):5903-12

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Human dendritic cells are activated by dengue virus infection: enhancement by gamma interferon and implications for disease pathogenesis.

Libraty DH, Pichyangkul S, Ajariyakhajorn C, Endy TP, Ennis FA
Journal of virology 2001 Apr;75(8):3501-8

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Generation and cytotoxic profile of human peripheral blood CD4+ T lymphocytes.

Smyth MJ
Immunology and cell biology 1992 Dec;70 ( Pt 6):379-90

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Direct ELISA analysis of TNF alpha was performed by coating wells of a 96-well plate with 100 µL per well of human recombinant protein TNF alpha (Product # RTNFAI) diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer (Product # 37538), and incubated with 100 µL per well of a mouse anti-TNF alpha monoclonal antibody (Product # M2TNFAI) at a dilution of 1:25, 1:50, 1:100, 1:200, 1:400, 1:800, 1:1600 and 1:3200 for 90 minutes at 37C. The plate was washed, then incubated with 100 µL per well of a HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 62-6520) at a dilution of 1:2000 for 90 minutes at 37C. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 5-10 minutes at room temperature in the dark. The reaction was stopped with Stop solution (Product # N600), and absorbances were read on a spectrophotometer at 450-550 nm.