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- Antigen structure
- References [3]
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- Product number
- M3TNFAI - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNF alpha Monoclonal Antibody (E10041)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M3TNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Canine, Feline, Human, Non-human primate, and Porcine samples.
- Antibody clone number
- E10041
- Concentration
- 0.5 mg/mL
Submitted references Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.
Expression of inflammatory markers in pig amnion after intraamniotic infection with nonpathogenic or enteropathogenic Escherichia coli.
Expression of inflammatory markers in pig amnion after intraamniotic infection with nonpathogenic or enteropathogenic Escherichia coli.
Ullah M, Liu DD, Rai S, Razavi M, Concepcion W, Thakor AS
Stem cell research & therapy 2020 Sep 14;11(1):398
Stem cell research & therapy 2020 Sep 14;11(1):398
Expression of inflammatory markers in pig amnion after intraamniotic infection with nonpathogenic or enteropathogenic Escherichia coli.
Splíchalová A, Splíchal I, Trebichavský I, Hojná H
Folia microbiologica 2004;49(6):751-6
Folia microbiologica 2004;49(6):751-6
Expression of inflammatory markers in pig amnion after intraamniotic infection with nonpathogenic or enteropathogenic Escherichia coli.
Splíchalová A, Splíchal I, Trebichavský I, Hojná H
Folia microbiologica 2004;49(6):751-6
Folia microbiologica 2004;49(6):751-6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Direct ELISA analysis of TNF alpha was performed by coating wells of a 96-well plate with 100 µL per well of human recombinant protein TNF alpha (Product # RTNFAI) diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer (Product # 37538), and incubated with 100 µL per well of a mouse anti-TNF alpha monoclonal antibody (Product # M3TNFAI) at a dilution of 1:25, 1:50, 1:100, 1:200, 1:400, 1:800, 1:1600 and 1:3200 for 90 minutes at 37C. The plate was washed, then incubated with 100 µL per well of a HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 62-6520) at a dilution of 1:2000 for 90 minutes at 37C. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 5-10 minutes at room temperature in the dark. The reaction was stopped with Stop solution (Product # N600), and absorbances were read on a spectrophotometer at 450-550 nm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Inflammatory cytokines. a Immunohistochemistry staining for inflammatory markers TNF-alpha and IL-6 in kidney tissue. b Western blot on kidney tissue measuring inflammatory markers TNF-alpha and NF-kappaB (left), alongside their quantification (right). c Quantitative real-time PCR on kidney tissue measuring inflammatory markers Nfkb , Il6 , and Tnfa . d ELISA measurement of blood serum concentrations of cytokines IL-1beta, IL-6, and TNF-alpha. Each group has n = 5 mice. Significant difference a p < 0.05: relative to untreated control; b p < 0.05: relative to AKI; c p < 0.05: relative to AKI-EV; d p < 0.05: relative to AKI-EV-pFUS
