Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB510-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat TNF-alpha in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse (rm) TNF-alpha and less than 0.2% cross-reactivity with rhTNF-alpha , rpTNF-alpha , and rhTNF-beta . In Western blots, this antibody shows 100% cross-reactivity with rmTNF-alpha and no cross-reactivity with rfeTNF-alpha .
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Rat TNF-alpha Antibody. Recombinant Rat TNF-alpha (Catalog # 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rat TNF-alpha (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF-alpha Monoclonal Antibody (Catalog # MAB510). The ND50 is typically 10-40 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).