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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB5670 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rabbit TNF-alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant TNF-alpha from cotton rat, cat, cow, dog, guinea pig, horse, human, mouse, pig, rat, or rhesus is observed. No cross-reactivity with recombinant human (rh) 4-1BB Ligand, rhAPRIL, rhBAFF, rhEDA, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1 beta 2, rhLymphotoxin alpha 2 beta 1, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, rhVEGI, recombinant mouse (rm) 4-1BB Ligand, rmBAFF, rmFas Ligand, rmGITR Ligand, rmLIGHT, rmOX40 Ligand, rmTRAIL, rmTRAIL-2L, rmTRANCE, or rmTWEAK is observed.
- Reactivity
- Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Rabbit TNF-alpha Antibody. Recombinant Rabbit TNF-alpha (Catalog # 5670-TG) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Rabbit TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rabbit TNF-alpha Monoclonal Antibody (Catalog # MAB5670). The ND50 is typically 0.4-2.4 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
