Explore
Validate
Learn
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB1507 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects canine TNF-alpha in direct ELISAs. In direct ELISAs, 25-100% cross-reactivity with recombinant human TNF-alpha, recombinant porcine TNF-alpha, and recombinant rhesus macaque TNF-alpha is observed.
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P51742- Isotype
- IgG
- Antibody clone number
- 236812
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A Vaccine Therapy for Canine Visceral Leishmaniasis Promoted Significant Improvement of Clinical and Immune Status with Reduction in Parasite Burden.
Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
Roatt BM, Aguiar-Soares RD, Reis LE, Cardoso JM, Mathias FA, de Brito RC, da Silva SM, Gontijo NF, Ferreira SA, Valenzuela JG, Corrêa-Oliveira R, Giunchetti RC, Reis AB
Frontiers in immunology 2017;8:217
Frontiers in immunology 2017;8:217
Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
Figueiredo MM, Amorim IF, Pinto AJ, Barbosa VS, Pinheiro Lde J, Deoti B, Faria AM, Tafuri WL
BMC immunology 2013 May 14;14:22
BMC immunology 2013 May 14;14:22
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF-alpha in Canine PBMCs. TNF-alpha was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) untreated or treated with calcium ionomycin and PMA using Mouse Anti-Canine TNF-alpha Monoclonal Antibody (Catalog # MAB1507) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Canine TNF-alpha Antibody. Recombinant Canine TNF-alpha (Catalog # 1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Canine TNF-alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine TNF-alpha Monoclonal Antibody (Catalog # MAB1507). The ND50 is typically 1.25-5.0 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
