IC410F

antibody from R&D Systems

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC410F

Provider

R&D Systems

Product name

Mouse TNF-alpha Fluorescein-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse TNF-alpha .

Reactivity

Mouse

Host

Rat

Isotype

IgG

Antibody clone number

MP6-XT22

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Tumor necrosis factor-alpha does not modulate ischemia/reperfusion injury in naïve myocardium but is essential for the development of late preconditioning.

Dawn B, Guo Y, Rezazadeh A, Wang OL, Stein AB, Hunt G, Varma J, Xuan YT, Wu WJ, Tan W, Zhu X, Bolli R
Journal of molecular and cellular cardiology 2004 Jul;37(1):51-61

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of TNF-alpha in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line treated with anti-CD3/anti-CD28, PMA, and Calcium Ionomycin were stained with Rat Anti-Mouse TNF-alpha Fluorescein-conjugated Monoclonal Antibody (Catalog # IC410F, filled histogram) or isotype control antibody (Catalog # IC005F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.