Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF13662 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal PTP1B/PTPN1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects endogenous human PTP1B and does not cross-react with recombinant mouse PTP1B in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Absence of leptin resistance in platelets from morbidly obese individuals may contribute to the increased thrombosis risk in obesity.
Dellas C, Schäfer K, Rohm I, Lankeit M, Ellrott T, Faustin V, Riggert J, Hasenfuss G, Konstantinides S
Thrombosis and haemostasis 2008 Dec;100(6):1123-9
Thrombosis and haemostasis 2008 Dec;100(6):1123-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human PTP1B by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13662) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human PTP1B by Western Blot. Detection of Human PTP1B by Western Blot.