AF13662

antibody from R&D Systems

Targeting: PTPN1 PTP1B

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF13662

Provider

R&D Systems

Product name

Human PTP1B Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects endogenous human PTP1B and does not cross-react with recombinant mouse PTP1B in Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P18031


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Absence of leptin resistance in platelets from morbidly obese individuals may contribute to the increased thrombosis risk in obesity.

Dellas C, Schäfer K, Rohm I, Lankeit M, Ellrott T, Faustin V, Riggert J, Hasenfuss G, Konstantinides S
Thrombosis and haemostasis 2008 Dec;100(6):1123-9

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human PTP1B by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13662) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Western Blot Shows Human PTP1B Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PTP1B knockout HeLa cell line (KO). PVDF membrane was probed with 0.3 µg/mL of Goat Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13662) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP1B at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human PTP1B by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13662) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.