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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP1-18928 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-18928, RRID:AB_1625578
- Product name
- Rabbit Polyclonal BAT3/BAG6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BAT3/BAG6 Antibody [NBP1-18928] - Whole cell lysate from HeLa cells. BAT3 was also immunoprecipitated by rabbit anti-BAT3 antibody NBP1-18929.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BAT3/BAG6 Antibody [NBP1-18928] - Detection of Human BAT3 by Western Blot. Samples: Whole cell lysate (50, 5 and 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BAT3 antibody NBP1-18928 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BAT3/BAG6 Antibody [NBP1-18928] - Detection of human BAT3 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BAT3 antibody NBP1-18928 used for IP at 6 ug per reaction. BAT3 was also immunoprecipitated by rabbit anti-BAT3 antibody NBP1-18927. For blotting immunoprecipitated BAT3, NBP1-18928 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
