Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB5466 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal KLF2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human KLF2 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross-reactivity with rhKLF1, 4, 5, 6, 10, 12, 17, recombinant mouse KLF4 or 15 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Memantine protects against ischemia/reperfusion-induced brain endothelial permeability.
Cellular origin and pathophysiology of chronic lymphocytic leukemia.
Liu Y, Huang Y, Xu Y, Qu P, Wang M
IUBMB life 2018 Apr;70(4):336-343
IUBMB life 2018 Apr;70(4):336-343
Cellular origin and pathophysiology of chronic lymphocytic leukemia.
Seifert M, Sellmann L, Bloehdorn J, Wein F, Stilgenbauer S, Dürig J, Küppers R
The Journal of experimental medicine 2012 Nov 19;209(12):2183-98
The Journal of experimental medicine 2012 Nov 19;209(12):2183-98
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of KLF2 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 Monoclonal Antibody (Catalog # MAB5466, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.