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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- AF1224 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PD-L2/B7-DC Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human PD-L2/B7-DC in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PD-L2 is observed and less than 1% cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b and recombinant rat B7-1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9BQ51- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mesenchymal Stromal Cell Secretion of Programmed Death-1 Ligands Regulates T Cell Mediated Immunosuppression.
PD-L1, PD-L2 and PD-1 expression in metastatic melanoma: Correlation with tumor-infiltrating immune cells and clinical outcome.
12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells.
Davies LC, Heldring N, Kadri N, Le Blanc K
Stem cells (Dayton, Ohio) 2017 Mar;35(3):766-776
Stem cells (Dayton, Ohio) 2017 Mar;35(3):766-776
PD-L1, PD-L2 and PD-1 expression in metastatic melanoma: Correlation with tumor-infiltrating immune cells and clinical outcome.
Obeid JM, Erdag G, Smolkin ME, Deacon DH, Patterson JW, Chen L, Bullock TN, Slingluff CL
Oncoimmunology 2016;5(11):e1235107
Oncoimmunology 2016;5(11):e1235107
12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells.
Jang BC, Park YK, Choi IH, Kim SP, Hwang JB, Baek WK, Suh MH, Mun KC, Suh SI
International journal of oncology 2007 Dec;31(6):1439-47
International journal of oncology 2007 Dec;31(6):1439-47
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human PD-L2/B7-DC by Western Blot. Western blot shows lysates of human lung tissue and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-L2/B7-DC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1224) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD-L2/B7-DC at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
