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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [3]
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Validation data
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- Product number
- TA802840 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA802840, RRID:AB_2626536
- Product name
- CD63 mouse monoclonal antibody, clone OTI5D9 (formerly 5D9)
- Antibody type
- Monoclonal
- Description
- CD63 mouse monoclonal antibody, clone OTI5D9 (formerly 5D9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CD63
- Isotype
- IgG
- Antibody clone number
- OTI5D9
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD63 (RC201733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD63.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from MCF7 cell line by using anti-CD63 monoclonal antibody .(1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of living A549 cells,using anti-CD63 antibody(TA802840),(Red),compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201733 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CD63 antibody(TA802840), and then analyzed by flow cytometry.(1:100)
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of MCF-7 cells, using anti-CD63 antibody(TA802840),(Red), compared to isotype control,(green), and negative control (PBS),(Blue)(1:100)
- Validation comment
- FC
