PA5-17536

antibody from Invitrogen Antibodies

Targeting: CSNK1A1 CK1, CK1a, CK1alpha, CKIa, CKIalpha

Antibody data

Submit

Validation data

CSNK1A1 protein structure - PA5-17536 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CK1 was performed by loading 25 µg of whole cell lysates from HEK293T cells, treated with a control siRNA (left lane) or CK1 siRNA (right lane) for 48 hours, per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk in TBST for 30 minutes at room temperature. The membrane was probed with a CK1 polyclonal antibody (Product # PA5-17536) at a dilution of 1:500 overnight at 4C, washed in TBST, and probed with an HRP-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:2000 for 1 hour at room temperature. Detection was performed using ECL Substrate (Product # 32106). Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CK1 in lysates from Hela and NIH/3T3 cell lines using CK1 polyclonal antibody (Product # PA5-17536).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CK1 in lysates from Hela and NIH/3T3 cell lines using CK1 polyclonal antibody (Product # PA5-17536).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CK1 was achieved by transfecting HEK-293 cells with CK1 specific siRNAs (Silencer® select Product # s3625). Western blot analysis (Fig. a) was performed using whole cell extracts from the CK1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with CK1 Polyclonal Antibody (Product # PA5-17536, 1:1000 dilution) and chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CK1

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using anti-CK1 Polyclonal Antibody (Product # PA5-17536) and a 37 kDa band corresponding to CK1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HEK 293 (Lane 1), HeLa (Lane 2), HCT 116 (Lane 3), MCF-7 (Lane 4) and A549 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).