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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB6555 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6555, RRID:AB_1574645
- Product name
- EIF4ENIF1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of EIF4ENIF1.
- Antigen sequence
C-AKVISVDELEYRQ- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E.
Dostie J, Ferraiuolo M, Pause A, Adam SA, Sonenberg N
The EMBO journal 2000 Jun 15;19(12):3142-56
The EMBO journal 2000 Jun 15;19(12):3142-56
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EIF4ENIF1 polyclonal antibody (Cat # PAB6555) staining (0.25 ug/mL) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EIF4ENIF1 polyclonal antibody (Cat # PAB6555, 5 ug/mL) staining of paraffin embedded human pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
