PA5-89074

antibody from Invitrogen Antibodies

Targeting: SLC29A1 ENT1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89074

Provider

Invitrogen Antibodies

Product name

ENT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: ELSESAFGYF ITACAVIILT IICYLGLPRL EFYRYYQQLK LEGPGEQETK LDLISKGEEP RAGKEESGVS VSNSQPTNES HSIKAILKNI SVLAFSVCFI F; Positive Samples: U-87MG; Cellular Location: Apical cell membrane, Basolateral cell membrane, Cell membrane, Multi-pass membrane protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.31 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of U-87MG cells, using SLC29A1 Polyclonal antibody (Product # PA5-89074) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ENT1 in extracts of U-87MG cells using ENT1 Polyclonal Antibody (Product # PA5-89074) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ENT1 in paraffin-embedded rat brain using ENT1 Polyclonal Antibody (Product # PA5-89074) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ENT1 in paraffin-embedded rat brain using ENT1 Polyclonal Antibody (Product # PA5-89074) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ENT1 in paraffin-embedded rat kidney using ENT1 Polyclonal Antibody (Product # PA5-89074) at a dilution of 1:100.