Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-30635 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CECR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.66 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CECR1 using 30 µg of A) HeLa (B) HepG2 and C) HCT116 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CECR1 polyclonal antibody (Product # PA5-30635) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CECR1 in Various whole cell extracts (30 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with CECR1 Polyclonal antibody (Product # PA5-30635) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CECR1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CECR1 Polyclonal Antibody (Product # PA5-30635) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.